Molecular study of co-infection between giardia lamblia and helicobacter pylori in non-symptomatic children in Thi-Qar province, Iraq

الملخص EN

The current study was carried out in Thi-Qar Province which included a collection of stool samples from non-symptoms children.

The aim of the study was to detect of co-infection of Giardia.

lamblia with Helicobacter pylori in non-symptoms children.

Atotal 96 stool samples taken children were under five years of both sex and examined by PCR for detection of G.

lamblia.

The results showed that the percentage of positive samples was 32 positive and 64 negative.

The most infection was in females more than males.

The highest infection with G.

lamblia according to PCR was in rural more than in urban population.

The highest infection rate with G.

lamblia in the age group (1-12 months) , Patients and the lowest age group (37-48 months) .A total of 96 samples were examined by PCR for diagnosis of G.

lamblia also to detection of H.

pylori .

The results showed 32 positive samples with G.

lamblia and only 14 positive samples with H.

pylori.

The number of samples that have co-infection between G.

lamblia with H.

pylori is 13.

The BLAST results for DNA sequencing showed that isolation for G.

lamblia different with the isolates recorded at the National Center for Biotechnology (NCBI); therefore, these isolates were registered in NCBI as new isolates under the GenBank accession numbers, that including two isolates per microorganism.

G.

lamblia, isolate IQ No.1 (MN096738) and G.

lamblia co-infection with H.

pylori isolate IQ No.2 (MN096739).

The accessions number for isolates of H.

pylori are, seq1(MN115555) and H.

pylori co-infection with G.

lamblia isolates seq2 (MN115556).