Identification of Oligella ureolytica from formalin-embalmed cadavers

المؤلفون المشاركون

Bakir, Amin A.
Khidr, Pinar K.
Rahman, Lajan Q.

المصدر

ZANCO Journal of Pure and Applied Sciences

الناشر

جامعة صلاح الدين قسم النشر العلمي

تاريخ النشر

2017-08-31

دولة النشر

العراق

عدد الصفحات

6

التخصصات الرئيسية

الأحياء

الملخص الإنجليزي

Formalin is a widely used fixative for embalming of cadavers with a well recognized antimicrobial activity.

However, with improper preservation techniques some human pathogens such as Oligella ureolytica can overcome this feature of formalin.

The current study was performed to detect this microorganism and consequently prevent its risks to medical students and embalmers.

Swab and tissue samples were collected from lesions, intact area, and surrounding surfaces of three formalin embalmed cadavers from tutorial anatomy lab in the college of medicine - HMU.

Samples were subsequently transferred to nutrient, MacConkey, and blood agar then, purified colonies were subjected to biochemical tests and microscopic observation.

Accordingly, advanced automated instrument was applied to confirm identification.

The isolated microorganism was then inoculated under increasing formalin concentrations.

The genus Oligella ureolytica was identified which appeared as Gram negative, rod shape with singular arrangement under microscopic observation.

The biochemical tests revealed positive for urease, oxidase, catalase, and motility with no evidence for hemolysis.

The growth of Oligella ureolytica was significant at 1% and 2% and low at 5% and 10% formalin concentration but no growth at higher concentrations.There are many reasons to choose formalin as fixative for embalming.

However; it is not totally able to protect cadavers from decomposition by microorganisms like Oligella ureolytica.

Thus, possible risk of contamination for medical students and embalmers exists when a fixative of low concentration was used.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-1388876

• APA
• MLA
• AMA