Synergistic antimicrobial activity of silver nanoparticles (NPs)‎ with essential oils of Cupresus sempervirens and Astriscus graveolens against pathogenic bacteria strains

العناوين الأخرى

النشاطية المضادة للبكتيريا لتآزر جسيمات الفضة النانوية (AgNPs)‎ مع الزيوت الأساسية لنبات السرو و نبات الأستربكا ضد سلالات البكتيريا المسببة للأمراض

مقدم أطروحة جامعية

Abu Rayyan, Inas Ibrahim

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2018

الملخص الإنجليزي

The sophisticated investigation and growth of silver nanoparticles (AgNPs) is wide because of their substantial applications nowadays.

To find out and probe the potential in extracellular biosynthesis of silver nanoparticles (AgNPs) by Tritirachium oryzae W5H and to examine the antibacterial activity of biosynthesized AgNPs and its synergy with two essential oils (EO) of A.

graveolens and C.semiperverens as comparing with 12 antibiotics against five tested bacteria.

The colour of the culture filtrate with silver nitrate solution changed to intense brown after 72 h of incubation.

Synthesis of AgNPs was recorded by UV-Visible spectroscopy.

The UVVisible spectrum showed a peak at 425 nm corresponding to the Plasmon absorbance of the AgNPs.

The synthesized silver nanoparticles were characterized with energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle analyzer.

Notable synergy was seen between AgNPs and vancomycin, nitrofurantoin, chloramphenicol or tetracycline, against Pseudomonas aeruginosa, in the range between 2.4-fold to 9 fold, increase in zone diameter.

Similarly, AgNPs with gentamycin against E.

coli or tetracycline against P.

aeruginosa or ampicillin against S.

aureus or cefotaxime against S.

aureus caused increase in the fold area of inhibition by 1-3 fold.

Likewise, synergy was seen between AgNPs, EO of A.

graveolens and either cefotaxime, nitrufurantion or amoxicillin against P.

aeruginosa, by 10-fold, 3-fold and 10-fold, respectively, whereas trimethoprim, tetracycline or amoxicillin against E.

coli by a 1-6-fold.

The lowest synergy effect obtained was against E.

aerogenes (1.7-fold).

Similarly, AgNPs plus EO of C.

semiperverens and either with trimethoprim against S.

epidermidis and vancomycin against P.

aeruginosa, led to the superior enhancement up to 29 and 16.4, respectively.

The MIC of AgNPs ranged from 6.4–19.2 μg/mL.

When the NPs solutioncombined with the EO of A.

graveolens, the MIC values reduced from 6.4–19.2 μg/mL to 1.0-9.5 μg/ml.

However, as C.

sempervirens EO replaced the EO A.

graveolens, the MIC of AgNPs ranged between 3.20-28.7 μg/mL.

AgNPs and its synergistic potential for enhancement of the antibacterial activity showed significant antibacterial activity against all tested bacteria.

التخصصات الرئيسية

الأحياء

عدد الصفحات

54

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-1402426