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Gingival crevicular fluid infiltrating CD14+ monocytes promote inflammation in periodontitis
المؤلفون المشاركون
Raj, A. Thirumal
Baashin, Husam Ali
Faqih, Haytham Nasir
Faqih, Hammam Ibrahim
Khan, Samar Said
Maganur, Prabhadevi C.
Vyas, Nishant
Patil, Vikrant R.
Patil, Shankargouda
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 28، العدد 5 (31 مايو/أيار 2021)، ص ص. 3069-3075، 7ص.
الناشر
تاريخ النشر
2021-05-31
دولة النشر
السعودية
عدد الصفحات
7
التخصصات الرئيسية
علم الاجتماع و الأنثروبولوجيا و الخدمة الاجتماعية
الملخص EN
Periodontitis is a condition that occurs because of inflammation-mediated tissue degeneration.
Many studies have been conducted to identify inflammatory molecules in periodontitis, but the well-defined role of cells from the immune system in the progression of periodontitis as well as in gingival tissue degeneration has not been appropriately established.
The objective of the present study was to charac- terize the monocytes isolated from the gingival crevicular fluid (GCF) in patients with periodontitis.
GCF was obtained from periodontitis patients and healthy controls.
Cytokine levels of CCL2 were evalu- ated by ELISA in GCF samples.
CD14+ monocytes were separated using magnetic sorting from GCF.
RT-qPCR was performed to assess the gene expression.
Cytometric bead array analysis was performed to analyze the levels of cytokines and chemokines in the secretome of cells.
CD14+ monocytes from GCF secreted higher levels of CCL2 and showed elevated expression of genes responsible for monocyte migration.
Additionally, upon lipopolysaccharide stimulation, these monocytes secreted higher levels of inflammatory cytokines and chemokines.
This investigation aids in understanding the inflammatory microenvironment of periodontitis by characterizing GCF in terms of infiltrated CD14+ monocytes, cytokines, and molecules secreted by these monocytes, which are specific for cellular differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faqih, Haytham Nasir& Faqih, Hammam Ibrahim& Khan, Samar Said& Maganur, Prabhadevi C.& Vyas, Nishant& Patil, Vikrant R.…[et al.]. 2021. Gingival crevicular fluid infiltrating CD14+ monocytes promote inflammation in periodontitis. Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.3069-3075.
https://search.emarefa.net/detail/BIM-1419244
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faqih, Haytham Nasir…[et al.]. Gingival crevicular fluid infiltrating CD14+ monocytes promote inflammation in periodontitis. Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.3069-3075.
https://search.emarefa.net/detail/BIM-1419244
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faqih, Haytham Nasir& Faqih, Hammam Ibrahim& Khan, Samar Said& Maganur, Prabhadevi C.& Vyas, Nishant& Patil, Vikrant R.…[et al.]. Gingival crevicular fluid infiltrating CD14+ monocytes promote inflammation in periodontitis. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.3069-3075.
https://search.emarefa.net/detail/BIM-1419244
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 3074-3075
رقم السجل
BIM-1419244
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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