SiRNA-mediated lncRNA ANRIL knockdown enhances the sensitivity of gastric cancer cells to doxorubicin
المؤلفون المشاركون
Chen, Jiahai
Dai, Xuejiang
Yu, Hebin
Chen, Lina
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 22، العدد 10 (31 أكتوبر/تشرين الأول 2020)، ص ص. 1-6، 6ص.
الناشر
تاريخ النشر
2020-10-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background: Long non-coding RNAs (lncRNAs) play pivotal roles in carcinogenesis and the development of drug resistance in various malignancies.
Objectives: The current study aimed to explore the impact of antisense non-coding RNA in the INK4 locus (ANRIL) silencing on proliferation and the sensitivity of KATO III gastric cells to the doxorubicin as a common chemotherapeutic agent.
Methods: The KATO III cells were transfected with ANRIL siRNA (si-ANRIL) using Lipofectamine™2000 reagent.
Following that, the relative ANRIL levels were determined by quantitative Real-Time PCR.
Trypan blue assay was conducted to observe the tumor cell proliferation following the transfection.
Moreover, the MTT assay was performed to identify the cytotoxic effects of doxorubicin and si-ANRIL alone or in combination on KATO III cells.
The effects of si-ANRIL/doxorubicin on KATO III cells migration and apoptosis were assessed by wound healing assay and ELISA cell death method, respectively.
Results: The results showed that the si-ANRIL significantly diminished ANRIL expression level in a time-dependent manner contributing to the distinct repression of cell growth and enhanced apoptosis.
Furthermore, the si-ANRIL synergistically elevated the cytotoxic impacts of doxorubicin.
Additionally, the ANRIL down-regulation dramatically promoted its induction of apoptosis.
Moreover, KATO III cells transfected with si-ANRIL and exposed with doxorubicin revealed significantly reduced invasion capability and enhanced apoptosis rate.
Conclusion: These results demonstrated that the knock-down of lncRNA ANRIL could be a potential therapeutic strategy to trigger apoptosis and circumvent doxorubicin-resistance.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Jiahai& Dai, Xuejiang& Yu, Hebin& Chen, Lina. 2020. SiRNA-mediated lncRNA ANRIL knockdown enhances the sensitivity of gastric cancer cells to doxorubicin. Iranian Red Crescent Medical Journal،Vol. 22, no. 10, pp.1-6.
https://search.emarefa.net/detail/BIM-1430678
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Hebin…[et al.]. SiRNA-mediated lncRNA ANRIL knockdown enhances the sensitivity of gastric cancer cells to doxorubicin. Iranian Red Crescent Medical Journal Vol. 22, no. 10 (Oct. 2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1430678
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Jiahai& Dai, Xuejiang& Yu, Hebin& Chen, Lina. SiRNA-mediated lncRNA ANRIL knockdown enhances the sensitivity of gastric cancer cells to doxorubicin. Iranian Red Crescent Medical Journal. 2020. Vol. 22, no. 10, pp.1-6.
https://search.emarefa.net/detail/BIM-1430678
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5-6
رقم السجل
BIM-1430678
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر