Comparison of performance characteristics of two immunoassays in the detection of antigenic components of plasmodium falciparum in blood from yemeni patients
العناوين الأخرى
مقارنة كفاءة مقايستين مناعيتين لتحري مستضدات المتصورة المنجلية في دم المرضى اليمنيين
المصدر
Journal of the Arab Board of Health Specializations
العدد
المجلد 6، العدد 4 (31 ديسمبر/كانون الأول 2004)، ص ص. 372-376، 5ص.
الناشر
المجلس العربي للاختصاصات الصحية
تاريخ النشر
2004-12-31
دولة النشر
سوريا
عدد الصفحات
5
التخصصات الرئيسية
الملخص AR
Objectives .: The purpose of the study was to compare the performance characteristics of two immunochromatographic antigen detection systems for the diagnosis of Plasmodium falciparum in an attempt to detect the parasite components in blood samples from Yemeni patients.
Methods: The traditional microscopic examination was performed to identify the cases of malaria and subsequently two immunoassays were compared: OptiMAL (R) detects parasite lactate dehydrogenase (pLDH) and Core ™ detects parasite histidine rich protein-2 (pHRP-2) in blood samples using monoclonal antibodies.
During the study period, blood samples from 518 cases of suspected malaria were collected from hospitals in Taiz (214) and Hodeidah (304) and examined microscopically.
All cases that were found positive for falciparum malaria were subsequently examined by the indicated immunoassays.
In addition, 87 blood samples from healthy individuals and 47 from subjects with heterologous infections were tested by the same immunoassays in order to assess sensitivity and specificity.
Results: Of 518 cases suspected of malaria, 171 (33%) were positive for trophozoites and/or gametocytes in the blood films examined.
Among them, the rate of infection in Taiz was 27% and in Hodeidah 37%, (P= 0.02).
Of 171 positive blood samples, 164 were positive by the OptiMAL immunoassay giving 96% sensitivity.
Specificity of this test was 100% as none of the heterologous infections was positive.
The Core immunoassay was able to detect pHRP-2 in 159 of 171 tested blood samples to give a 93% sensitivity.
This test showed cross reactivity with blood samples from patients with toxoplasmosis giving 94% specificity.
Nineteen of the parasitologically confirmed cases were classified as negative by the two immunoassays (7 with OptiMAL and 12 with Core).
Conclusion: The newer generation antigen detection systems have a high degree of sensitivity and specificity.
This makes such systems a promising tool for diagnosis of falciparum malaria in Yemen.
الملخص EN
Objectives .: The purpose of the study was to compare the performance characteristics of two immunochromatographic antigen detection systems for the diagnosis of Plasmodium falciparum in an attempt to detect the parasite components in blood samples from Yemeni patients.
Methods: The traditional microscopic examination was performed to identify the cases of malaria and subsequently two immunoassays were compared: OptiMAL (R) detects parasite lactate dehydrogenase (pLDH) and Core ™ detects parasite histidine rich protein-2 (pHRP-2) in blood samples using monoclonal antibodies.
During the study period, blood samples from 518 cases of suspected malaria were collected from hospitals in Taiz (214) and Hodeidah (304) and examined microscopically.
All cases that were found positive for falciparum malaria were subsequently examined by the indicated immunoassays.
In addition, 87 blood samples from healthy individuals and 47 from subjects with heterologous infections were tested by the same immunoassays in order to assess sensitivity and specificity.
Results: Of 518 cases suspected of malaria, 171 (33%) were positive for trophozoites and/or gametocytes in the blood films examined.
Among them, the rate of infection in Taiz was 27% and in Hodeidah 37%, (P= 0.02).
Of 171 positive blood samples, 164 were positive by the OptiMAL immunoassay giving 96% sensitivity.
Specificity of this test was 100% as none of the heterologous infections was positive.
The Core immunoassay was able to detect pHRP-2 in 159 of 171 tested blood samples to give a 93% sensitivity.
This test showed cross reactivity with blood samples from patients with toxoplasmosis giving 94% specificity.
Nineteen of the parasitologically confirmed cases were classified as negative by the two immunoassays (7 with OptiMAL and 12 with Core).
Conclusion: The newer generation antigen detection systems have a high degree of sensitivity and specificity.
This makes such systems a promising tool for diagnosis of falciparum malaria in Yemen.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Azazy, Ahmad A.& Muhammad, Yasin A. K.& al-Shumayri, Shawqi A. M.. 2004. Comparison of performance characteristics of two immunoassays in the detection of antigenic components of plasmodium falciparum in blood from yemeni patients. Journal of the Arab Board of Health Specializations،Vol. 6, no. 4, pp.372-376.
https://search.emarefa.net/detail/BIM-143480
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Azazy, Ahmad A.…[et al.]. Comparison of performance characteristics of two immunoassays in the detection of antigenic components of plasmodium falciparum in blood from yemeni patients. Journal of the Arab Board of Health Specializations Vol. 6, no. 4(December 2004), pp.372-376.
https://search.emarefa.net/detail/BIM-143480
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Azazy, Ahmad A.& Muhammad, Yasin A. K.& al-Shumayri, Shawqi A. M.. Comparison of performance characteristics of two immunoassays in the detection of antigenic components of plasmodium falciparum in blood from yemeni patients. Journal of the Arab Board of Health Specializations. 2004. Vol. 6, no. 4, pp.372-376.
https://search.emarefa.net/detail/BIM-143480
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 376
رقم السجل
BIM-143480
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر