Isolation of Jatropha Curcas seeds isolectins with variable affinity for human and animal blood types
المؤلفون المشاركون
al-Ghali, Abir
Ahmad, Rim M.
Konozy, Imad al-Din H. E.
Mudawi, Jad Allah
المصدر
Sudan Journal of Medical Sciences
العدد
المجلد 14، العدد 4 (31 ديسمبر/كانون الأول 2019)، ص ص. 202-209، 8ص.
الناشر
جامعة أم درمان الإسلامية كلية الطب
تاريخ النشر
2019-12-31
دولة النشر
السودان
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background: Lectins are carbohydrate-binding protein which agglutinate glycoconjugates in a reversible way, they are with wide applications in biological and medical sciences.
Jatropha curcas belongs to the family euphorbiaceae and is distributed in many tropical and subtropical countries.
The toxicity of this plant is known for long ago and has been attributed to several components among which is a protein called curcin.
Methods: Jatropha curcas seeds were pulverized and protein was extracted with suitable buffer.
Protein extract thus obtained had undergone successive protein precipitations by salting-out using (NH4)2SO4 (AS) at 40, 60, and 80% saturations.
Lectin activity was detected by hemagglutination method using human- and animal blood types.
AS-precipitated protein fractions that possess lectin activity were tested for their antimicrobial activity against the pathogenic Staphylococcus aureus, Escherichia coli, Bacillus aueras, and Candida albicans.
Results: At least three isolectins (Lec40, Lec60, and Lec80) were detected by hemagglutination (HA) and isolated by AS fractionation from the crude Jatropha curcas seed extract (CExt).
The isolectins exhibited different tendency toward human and animal blood types.
None of the isolectins could inhibit any of the used bacterial strains and Candida albicans.
Conclusions: In this study, though the detected lectins resemble their counterpart legume lectins, they, however, showed apparently unique and variable behavior toward human and animal blood types.
Which might emphasize on the need for further structural analysis on the affinity sites of these proteins.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Ghali, Abir& Mudawi, Jad Allah& Ahmad, Rim M.& Konozy, Imad al-Din H. E.. 2019. Isolation of Jatropha Curcas seeds isolectins with variable affinity for human and animal blood types. Sudan Journal of Medical Sciences،Vol. 14, no. 4, pp.202-209.
https://search.emarefa.net/detail/BIM-1435876
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Ghali, Abir…[et al.]. Isolation of Jatropha Curcas seeds isolectins with variable affinity for human and animal blood types. Sudan Journal of Medical Sciences Vol. 14, no. 4 (2019), pp.202-209.
https://search.emarefa.net/detail/BIM-1435876
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Ghali, Abir& Mudawi, Jad Allah& Ahmad, Rim M.& Konozy, Imad al-Din H. E.. Isolation of Jatropha Curcas seeds isolectins with variable affinity for human and animal blood types. Sudan Journal of Medical Sciences. 2019. Vol. 14, no. 4, pp.202-209.
https://search.emarefa.net/detail/BIM-1435876
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 207-209
رقم السجل
BIM-1435876
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر