Beneficial effects of intrathecal injection of methylprednisolone against spinal cord injury in rats
المؤلفون المشاركون
Zhang, Zhe
Guan, Zhengmao
Wang, Cong
Ma, Hui
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 22، العدد 6 (30 يونيو/حزيران 2020)، ص ص. 1-8، 8ص.
الناشر
تاريخ النشر
2020-06-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background: High dose intravenous (i.t.) injection of methylprednisolone (MPSS) for spinal cord injury (SCI) is clinically contro- versial.
Objectives: This study aimed to investigate whether i.t.
MPSS would have a beneficial effect on SCI and whether or not it is a safe operation for SCI patients.
Methods: An animal experiment was conducted to explore the safety and feasibility of i.t.
Administration of MPSS.
Male Sprague- Dawley rats were randomly divided into four groups: (1) sham group, i.t.
injection of normal saline (NS) (n = 25); (2) control group, SCI surgery (created using the Infinite Horizon IH-400 impactor) with i.t.
injection of NS (n = 25); (3) i.t.
MPSS1 group, SCI with i.t.
injection of MPSS by a pulse therapy (n = 25); (4) i.t.
MPSS2 group, SCI with i.t.
injection of MPSS intermittently (n = 25).
Malondialde- hyde (MDA), superoxide dismutase (SOD), and inflammatory cytokines in serum were measured at 6h, 24h, 48h, 7d, and 14d after surgery with commercial assay kits.
Glial fibrillary acidic protein (GFAP) level was observed at 14 days after surgery by immunohis- tochemistry.
Motor evoked potentials (MEP) and somatosensory evoked potential (SEP) were monitored and recorded separately before surgery and 1, 7, and 14 days after surgery.
Also, locomotor function was evaluated using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale.
Results: The results showed that the levels of MDA and SOD, and three inflammatory cytokines, including IL-1b, IL-6, and TNF-α were reduced in i.t.
MPSS groups than that of the control group (all P < 0.05).
The expression of GFAP was inhibited after i.t.
MPSS treatment.
The amplitude was reduced, and the latency period of SEP and MEP recovery was prolonged (all P < 0.05) after MPSS administration.
In addition, the recovery of limb function (BBB score) was significantly ameliorated (P < 0.05) in SCI rats treated with MPSS compared with the control group.
Conclusions: Our results demonstrated that i.t.
MPSS was a potential strategy for reducing the secondary damage after SCI, espe- cially the MPSS pulse therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Cong& Guan, Zhengmao& Ma, Hui& Zhang, Zhe. 2020. Beneficial effects of intrathecal injection of methylprednisolone against spinal cord injury in rats. Iranian Red Crescent Medical Journal،Vol. 22, no. 6, pp.1-8.
https://search.emarefa.net/detail/BIM-1437548
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Zhe…[et al.]. Beneficial effects of intrathecal injection of methylprednisolone against spinal cord injury in rats. Iranian Red Crescent Medical Journal Vol. 22, no. 6 (Jun. 2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1437548
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Cong& Guan, Zhengmao& Ma, Hui& Zhang, Zhe. Beneficial effects of intrathecal injection of methylprednisolone against spinal cord injury in rats. Iranian Red Crescent Medical Journal. 2020. Vol. 22, no. 6, pp.1-8.
https://search.emarefa.net/detail/BIM-1437548
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8-9
رقم السجل
BIM-1437548
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر