Isolation and molecular detection of Acanthamoeba spp from environmental sources in Khartoum-Sudan
المؤلفون المشاركون
Lawati, Jamal Yusuf
Sulayman, Hamid
Sati, Abd al-Salam Bashir
Muhammad, al-Nazir
المصدر
Sudan Medical Laboratory Journal
العدد
المجلد 9، العدد 1 (31 ديسمبر/كانون الأول 2021)، ص ص. 44-50، 7ص.
الناشر
جامعة أم درمان الإسلامية كلية المختبرات الطبية
تاريخ النشر
2021-12-31
دولة النشر
السودان
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
Acanthamoeba spp is infection of the human ey cornea by the free-living protozoan amoeba belonged to the genus Acanthamoeba ubiquitous in Varity of environments and can lead to sever disease including amoebic keratitis and granulomatous encephalitis.
Purpose: to isolates and to determine the most prevalent of Acanthamoeba Spp predominant in soil, hospital dust, stagnant waters, river water samples as risk factors transmission Acanthamoeba from Khartoum central Sudan.
Material and Methods : this is cross sectional study , a total of 70 environmental samples were collected and examinated for the present of Acanthamoeba in the period of time 2017-2020 we use a standard protocol for isolation , all samples after filtering through 0.45-um nitrocellulose paper were cultured in 1.5 % non –nutrient agar medium enriched with heat-killed Escherichia coli for detecting and identification of Acanthamoeba species we employ cultures method based on morphological criteria of trophozoite and cyst stages and polymerase chain reaction using specific primers JDP1- JDP2 for Acanthamoeba detection .
Results: The results revealed that out of 8 (11.4% ) of environmental samples positive for Acanthamoeba species by microscopy and culture technique was derived from soil 1(10% ) , hospital dust 1(10% ), stagnant water samples 4 (13.3% ) ,and 2 (10%) river water samples were positive for Acanthamoeba spp ,which have been observed in all environmental isolates samples.
Conclusions : Environmental sources , soil ,hospital dust , stagnant water ,river water samples is potentially risk factors for the prevalent of Acanthamoeba spp in Khartoum region due to high resistance of cyst against harsh environments as well as tolerance of it to the relatively high temperature.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lawati, Jamal Yusuf& Sulayman, Hamid& Sati, Abd al-Salam Bashir& Muhammad, al-Nazir. 2021. Isolation and molecular detection of Acanthamoeba spp from environmental sources in Khartoum-Sudan. Sudan Medical Laboratory Journal،Vol. 9, no. 1, pp.44-50.
https://search.emarefa.net/detail/BIM-1437737
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lawati, Jamal Yusuf…[et al.]. Isolation and molecular detection of Acanthamoeba spp from environmental sources in Khartoum-Sudan. Sudan Medical Laboratory Journal Vol. 9, no. 1 (2021), pp.44-50.
https://search.emarefa.net/detail/BIM-1437737
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lawati, Jamal Yusuf& Sulayman, Hamid& Sati, Abd al-Salam Bashir& Muhammad, al-Nazir. Isolation and molecular detection of Acanthamoeba spp from environmental sources in Khartoum-Sudan. Sudan Medical Laboratory Journal. 2021. Vol. 9, no. 1, pp.44-50.
https://search.emarefa.net/detail/BIM-1437737
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 50-51
رقم السجل
BIM-1437737
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر