The optimum conditions for the production of invertase by local isolate escherichia coli from the soil and grown on different carbon source substrate

الملخص EN

The present study aims to use a local isolate of Escherichia coli for the production of invertase enzyme and study the effects of some factors that may enhance the production of this enzyme.

For this purpose, different nutrient requirements and different culture conditions such as pH, temperature, carbon source, carbon concentration, inoculum volumes and incubation period were used.

The results revealed that the optimum pH, temperature, and incubation period were 7.0, 30 ℃ and 72 hours, respectively.

The red carrot, as a substrate, was the best carbon source for Escherichia coli at the concentration of 2.5% to get highest enzyme production up to 2.4 U/ml