التأثير التثبيطي للمواد الفينولية المستخلصة من أوراق السدر و السعف تجاه أنزيم الفا-أميليز

الملخص EN

This study was conducted to detect the inhibitory effect of in water and alcoholic extract on α-Amylase activity.

The extracts were prepared by mixing leaves powder with water (1:4 w/v) and with alcoholic (1:5 w/v), Both mixtures were placed at shaking incubater and left for 24 hr for water extract and 72 hr for alcohol extract.

The result of phenolic compound analysis showed that the alcoholc extract contained (40, 26.4mg/g), while the water extract contained (31.8 and 25.7 mg/g) respectively.

The results of separation and identification of the Phenolic compounds in sidr and palm leaf by using HPLC technology showed the presence of Gallic, Caffic, Ferulicand P-coumaric in concentration (0.838, 2.560, 4.931, and 0.615) µg/gm in Ziziphus spina–christ respectively and (0.485, 1.592, 0.656, and 0.438) µg/gm in Phoenix dactylifera respectively.

The result of the inhibition Activity of Phenolic compounds in water and alcoholic extracts when used at different concentrations (0.2, 0.4, 0.6, 0.8, and 1) mg/ml showed the superiority of alcoholic extract of Ziziphus spina–christ leaves in the percentage of inhibition which reached 45.32% were 1.0 mg/ml was used, while alcoholic extract of phoenix dactylifera leaves recorded 23.75%.

The water extract of ziziphus spina – christ and phoenix dactylifera leaves recorded a percentage of inhibition about 31.30% and 23.0% respectively.