Identification of mycobacterium avium subspecies paratuberculosis in faecal and tissue smears from small ruminants

العناوين الأخرى

التعرف على المتفطرة الطيرية النوع الفرعي نظيرة السلية في مسحات روث و أنسجة من مجترات صغيرة

المؤلفون المشاركون

Quraysh, Imhammad Khalifah
Mansur, Layla al-Hadi
al-Khalab, Ibtisam Miftah
al-Qadi, Ahmad Sayd
al-Atrish, Sulayman Mustafa
Duwayb, Ahmad Umar

المصدر

مجلة جامعة مصراتة للعلوم الزراعية

الناشر

جامعة مصراتة كلية الزراعة

تاريخ النشر

2021-12-31

دولة النشر

ليبيا

عدد الصفحات

11

التخصصات الرئيسية

الطب البيطري

الملخص الإنجليزي

Paratuberculosis (Johne’s disease) is a contagious chronic incurable disease of ruminants, can reduce the productivity, and difficult to diagnose and control.

It is caused by Mycobacterium avium sub species paratuberculosis (MAP).

Ruminant paratuberculosis is pathologically similar to human inflammatory bowel disease (IBD) which includes three pathological forms; Crohn’s disease (CD), ulcerative colitis (UC) and indeterminate colitis or unclassified IBD.

The zoonotic concern is much to be detained since MAP is heat resistant and is capable of hiding inside white blood cells.

In the current study the presence of acid-fast bacilli compatible with MAP in faecal and tissue smears were microscopically examined using Ziehl-Neelsen stain technique.

During the period from the 9th of November 2017 to 7th of March 2018, a total of 27 faecal smears and 14 tissues smears gathered from 12 sheep and 15 goats were tentative infected with paratuberculosis (depending on the clinical features).

These animals representing19 commercial herds in various locations in Northwestern Libya.

Eighteen (66.6%) faecal smears demonstrated clumps compatible with acid-fast bacilli.

Ten animals (71.4%) were positive depending on the presence of the same microorganism in tissue smears.

Moreover, positivity of combination of both faecal and tissue smears was 85.7%.

Additionally, our results indicate the importance of ileocecal lymph node as a target tissue for the detection of MAP.

The findings of the present study reveal that Ziehl-Neelsen stain procedure is indicated for the rapid identification of MAP existing in faeces and tissues.

Furthermore, this high occurrence showed a require for the application of a vigorous programme to control of small ruminants paratuberculosis, established on more sensitive tests, improving juvenile livestock administration, and rising biosecurity procedures.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-1457098

• APA
• MLA
• AMA