Cryopreservation storage of iris bostrensis and iris aurantica by encapsulation-dehydration for long-term

الملخص EN

Encapsulation-dehydration techniques of Iris bostrensis and Iris aurantica plants has been established for the first time in Syria.

Shoot-tips were excised from in vitro grown plants and incubated for 3 days on solid Murashige and Skoog (HF-MS) media supplemented with 0.3 M sucrose under complete darkness at 25±1°C.

The precultured shoot-tips were suspended in Hormone free-Murashige and Skoog media (HF-MS) containing 3% sodium alginate and 0.3 M sucrose for 20 minutes in which calcium was omitted from all media components.

The water content of beads decreased during preculture from 78% to 23% and 17% after 4 and 6 h of dehydration, respectively.

survival percentage increased rapidly from 67% (unacclimated shoot tips) to (77, 82%) after 4, 8 weeks of cold acclimation, respectively, with significant difference compared to the control (unacclimated shoot tips), and the best recovery percentage was 39% after 8 weeks with significant difference compared to the control.

The Genetic stability studied by using 22 ISSR primers were used in this study.

The genetic stability was high (from 95 to 99%) before and after storage in Liquid Nitrogen of Iris bostrensis.

Therefore, storage of Iris bostrensis, Iris aurantica in Liquid Nitrogen was found to have no adverse effect (genetic variation) on the regeneration rates.