Biochemical changes of skin cancer mice model induced by dmba and treated with melatonin and nigella sativa extract (role of apoptosis and oxidative stress)‎

الملخص EN

Skin cancer is the most common form of cancer in the United States with more than one million cases diagnosed yearly leading to an estimated 10,850 deaths in 2007.

Skin cancers represent a major public health problem worldwide, with increasing workload and cost in dealing with this problem, together with significant morbidity suffered by those affected.

Melatonin and Nigella sativa are substances with anticancer effects.

To date, our understanding of the mechanisms of therapeutic effects of these products in skin cancer is still marginal.

To look at the therapeutic values of these products, we carried out this investigation.

An animal model formed of 24 male mice was established.

The animals were divided into four groups of 6 animals each divided as the follow: (a) Control group Received 0.5-ml saline orally; (b) carcinogenic group: each animal of the carcinogenic groups was applied locally by a single dose of DMBA.

(c) MEL- treated group: Each animal of MEL-treated group was treated locally with MEL.

(d) NS- treated group: Each animal ofNs-treated group was treated locally with Ns.

The animals were killed, and their tissues were evaluated for (a) marker of apoptosis (tissue caspase-3 activity) (b) markers of oxidative stress (tissue lipid peroxide and tissue nitric oxide).