Circulating endothelial and platelet microparticles in end-stage renal disease patients treated with hemodialysis

الملخص EN

Introduction and Objectives : End-stage renal disease (ESRD) patients have high incidence of thrombotic events.

The pathogenesis of the thrombophilic tendency in those patients is not clearly defined.

Endothelial dysfunction and/or platelet activation may have an important role in thrombosis in ESRD.

Circulating endothelial micro particles (EMPs) are circulating small fragments of plasma membranes of activated endothelial cells.

Increased levels of circulating activated platelets and platelet hyperaggregability have been described in ESRD patients.

Circulating platelet micro particles (PMPs) arc small vesicles with procoagulant activity released from activated platelets.

The aim of this study was to determine the levels of both circulating EMPs and PMPs in ESRD patients under maintenance hem dialysis therapy.

Methodology : Circulating levels of both EMPs and PMPs were measured by flow cytomclry in phitelel-poor plasma of 25 hem dialysis patients younger than 40 years old (14 females and 11 males) and 20 age-matched healthy controls.

The blood samples were taken from the venous line before the start of the dialysis session.

All patients were subjected to full history taking and clinical examination.

Patients known to have any of the disease conditions that is known to cause endothelium and / or platelet activation (Diabetes mellitus, systemic lupus erythematosus, cerebrovascular or ischemic heart disease) were excluded.

Results : The level of EMPs was higher in dialysis patients (45.20 ± 11.03) than in (he control subjects (25.2 ± I3.13) with a statistically significant difference between the two groups fp = 0.002].

Also, the level of PMPs showed a statistical significant difference [p = 0.01] between dialysis patients and the control group (755.0 ± 187.9 vs 576.0 ± 117.70).

In the dialysis group, the EMPs counts were negatively correlated with platelet counts (r = -0.41 ; p = 0.04) but were positively correlated with the pre-dialysis diastolic blood pressure (r = 0.34 ; p = 0.02), while the levels of PMPs were negatively correlated with the hemoglobin levels in the dialysis group (r = -0.41; p= 0,04).

The levels of PMPs were positively correlated with the prc-dialysis systolic blood pressure (r = 0.34; p = 0.02) and the duration of hem dialysis therapy (r = 0.4; p = 0.05).

Conclusions : Increased concentrations of both endothelial-derived (EMPs) and platelet-derived (PMPs) were detected in hem dialysis patients.

This may indicate endothelium and platelet activation or injury in ESRD patients.

Further large scale studies are needed to confirm their roles in thrombotic events and their clinical implications.