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Determination of microcarrier radius that can be cultured in a rotational wall vessel bioreactor
المؤلف
المصدر
The Egyptian Journal of Medical Human Genetics
العدد
المجلد 10، العدد 2 (30 نوفمبر/تشرين الثاني 2009)، ص ص. 208-217، 10ص.
الناشر
الجمعية المصرية للأمراض الوراثية
تاريخ النشر
2009-11-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Introduction: Bioreactor are used as cell culture systems for growth of tissue – engineered, because strong shear stress effect on bioreactor damages delicate cells and is hypothesized to degrade the formation of three-dimensional tissue-like structures, such a stress should be analyzed.
In this study, the forces acting on a small piece of tissue or a microcarrier particle and its movement in the RWV bioreactor with inner cylinder were analyzed.
The tracks of a particle in RWV bioreactor were calculated under different inner and outer cylinder rotating speeds, different particle sizes.
The shear stress acting on the particle was analyzed.
Finally, an expression between the shear stress acting on the particle and the microcarrier radius is obtained.
Objectives: Determination of the range of microcarrier radius or tissue size, which could be safely cultured in the RWV bioreactor.
Materials and Methods: The forces of rotational wall vessel (RWV) bioreactor on small tissue pieces or microcarrier particles was calculated and the shear stress acting on the particle was analyzed.
Results: The range of microcarrier radius or tissue size, which could be safely cultured in the RWV bioreactor, in terms of shear stress level, was determined.
Conclusion: The upper safe size range for the particles cultured in RWV is 600-1300.
?m in radius.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Farraj, Shirin I.. 2009. Determination of microcarrier radius that can be cultured in a rotational wall vessel bioreactor. The Egyptian Journal of Medical Human Genetics،Vol. 10, no. 2, pp.208-217.
https://search.emarefa.net/detail/BIM-203766
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Farraj, Shirin I.. Determination of microcarrier radius that can be cultured in a rotational wall vessel bioreactor. The Egyptian Journal of Medical Human Genetics Vol. 10, no. 2 (Nov. 2009), pp.208-217.
https://search.emarefa.net/detail/BIM-203766
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Farraj, Shirin I.. Determination of microcarrier radius that can be cultured in a rotational wall vessel bioreactor. The Egyptian Journal of Medical Human Genetics. 2009. Vol. 10, no. 2, pp.208-217.
https://search.emarefa.net/detail/BIM-203766
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 215-217
رقم السجل
BIM-203766
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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