Oncogenic molecules in renal cell carcinoma, immunohistochemical study

الملخص EN

Background: This study was planned to assess the role of pVHL, MET and TFE3 in development, morphogenesis and diagnosis of different types of renal cell carcinomas (RCCs).

Also to assess if there is a link between the pathogen etic ways of these molecules.

Material and methods : we studied 59 selected cases of different RCCs types.

These were 24 clear cell RCCs (CRCC), 7 papillary RCCs type 1 (PRCC1), 5 papillary RCCs type 2 (PRCC2), 11 Xpll.2 translocation RCCs (XP11.2RCC), 9 homophobe RCCs (ChRCC) and 3 sarcomatoid RCCs (SRCC).

Paraffin-embedded sections were immunostained using primary antibodies against pVHL, MET and TFE3 ; the antigen antibody reaction was detected by a streptavidin-biotin kit.

Results : we found under expression of pVHL in all CRCC, all SRCC and 6 PRCC1.

MET was over expressed in 2 PRCC1 and 7 Xpll.2RCC.

Other tumor types showed under expression of MET except 1 SRCC.

TFE3 was detected in all Xpll.2RCC as strong nuclear staining.

Over expression of pVHL was detected in RCCs with papillary pattern.

No change in expressions of these molecules in ChRCC was detected.

A small correlation between the mean expressions of these molecules way found.

No correlation between expressions of these molecules and tumor behavior could be detected.

Moderate to strong correlation between tumor inflammatory cell infiltrate, tumor necrosis, Fuhrman grade and metastasis was noticed.

Conclusion : RCCs have different oncogenic pathways that interact in some points.

Such interaction may determine tumor phenotype and histological pattern.

Diagnosis of RCCs depends largely on histology ; however expression of these molecules contributes to the diagnosis.

Prognostic factors for RCCs include: tumor inflammation, necrosis and Fuhrman grade.