Induced M3 tolerant mutants of mungbean to calcareous soil in the basis of polypeptide subunits

الملخص EN

Three field experiments were conducted at Maryout Agricultural Research Station of Desert Research Center during 1995, 1996 and 1997 summer seasons and several Gel slabs of SDS-PAGE were prepared for selecting elite mungbean M3 mutants characterized by presence /absence of specific polypeptide patterns. Results revealed significant differences between genotypes for seed yield /plant and four of its attributes indicating that the selected M3 mutants are differentiated from each other and from its mother variety (Giza-1) as affected by calcareous soil conditions.

40 kr.

Gamma.

irradiation in the rate of 6.9 rad / sec) found to be more effective as inducing the highest seed yield / plant M3 mutant No.

3740 Significant positive association was found between yielding ability and each of; seed index (0.645**), flowering date (0.589**) and number of pods / plant (0.223*).

In the point of breeders view, these three attributed characters considered to be good selection criteria for improving mungbean tolerance to such experimental stresses.

Early flowering genotypes 120, 320 and 220 are characterized by the presence of 33.9, 33.4 and 18.0 KD polypeptide subunits and decrement in 78, 62, and 50 KD band intensities.

Also absence of 46.2, 40.6, 33.4 and 18 KD bands with high intensities in 78 and 50 KD polypeptide bands are found in the genotypes1720, 3740, 3940 and 5240 which gave heavier seeds.

In addition, genotypes with more number of pods per plant; 3230, 1620, 3030 and 3430 attained denser major bands (78, 62, 50, 44 and 32 KD) with absence of the minor bands (109, 33.4 and 13 KD).

Increment in major and intermediate band intensities is not always correlated with high yield or yield attributes as we observe with the number of seed per pod character, the higher intensities in bands 78, 62, 50, 44, 39 and 32 KD alongside with the genotypes with low number of seed per pod (3030, 3530 and 3230) also presence of 33.9, 33.4, 16 and 13 KD subunits.

Concerning seed yield / plant, the absences of 33.9, 33.4 and 13 KD components, in addition to high intensities of 62, 44 and 39 KD polypeptide bands result in increment of genotypes seed yield / plant.