Experimental clinicopathological studies on selenium toxicity in rats

الملخص EN

This work aimed to study the effect of increasing selenium on some hematological, biochemical and immunological parameters in addition to study their effects on some pathology in albino rats.

This study performed on 75 male albino rats, which divided into 3 equal groups (each one contains 25 rats).

The first group (control group), received no treatment.

The second group (Se1group), received low dose of selenium, 2ppm sodium selenite / liter in drinking water for 3 months.

The third group (Se2 group), received high dose of selenium, 4ppm sodium selenite / liter in drinking water for 3 months.

Collection of blood samples and separation of serum samples at the end of the 1st, 2nd and 3rd month of experiment for hematological, serum biochemical and immunological analysis.

Tissue specimens were collected at the end of the 1st, 2nd and 3rd months of experiment for histopathological examination.

Administration of high dose of selenium (4ppm) at the third group resulted in, significant decrease in live body weight of rats, anemia which was macrocytic hypochromic, also we recorded decrease in total leukocytic count with significant decrease in neutrophil count.

Regarding serum biochemical analysis we reported, significant elevation in ALT, AST, ALP and total bilirubin.

On the other hand there were significant decrease in total protein and albumin.

IL 1β was increased after 2months then decreased after 3months.

Hisopathological examination of tissue specimens showed serious lesions in liver, kidney, thymus, intestine and spleen.

It concluded that high doses of selenium revealed regenerative anemia, significant undesirable changes in serum biochemical and immunological parameters.

Also, there were multiple pathological lesions in different organs.