Determination of ascorbic acid via semi automated on –line merging zone technique

الملخص EN

A Flow injection analysis method for determination of ascorbic acid as Molybdenum blue is described.

Orthophosphate ion, Molybdenum and ascorbic acid were reacted to form molybdenum blue then the absorbance was measured at 660 nm.

The typical analytical working curve obtained under the optimized experimental conditions were rectilinear form (1 x 10-3_1x 10-4 mM) and the limit of detection was 11.4 ng .Measurement of one sample (without measurement time loading) takes 1minute.

The method setup offers a precise, sensitive, selective and high sample through put technique for determination of Ascorbic acid in standards sample.

It compared well with conventional standard (1) spectrophotometer method.