Differential pulse polarographic assay of azathioprine in serum and urine

الملخص EN

The polarographic behaviour of imuran (azathioprine) have been studied, the drug give a well sensitive defined double peaks at about (-0.4V, -0.96V) in carbonate buffer solution at pH 7.4 and at (-0.54V, -1.07 V) in serum–carbonate buffer and at (-0.42V, - 1.03 V) in urine–carbonate buffer.

The lowest determined concentration was (8.7 x 10-7 M) in serum-carbonate media and (15.2 x 10-7 M) in urine-carbonate media.

The method can be successfully applied to determination of azathioprine in vivo.

The relation between concentration and diffusion current (Ip) was linear for all solutions.