Spectroscopic studies of prostate specific antigen (PSA)‎ molecule

الملخص EN

Spectroscopic studies in the U.

V region were carried out on human PSA. The U.

V spectra of PSA, was obtained and revealed that PSA have a characteristic spectrum.

The effect of pH and polarity on PSA U.

V spectra was studied. Spectrophotometric pH titration and solvent perturbation were carried out and the data revealed that about 83.3 % of tyrosine residues are located on the surface of the PSA molecule, while about 16.7 % of tyrosine residues are buried interior the folded structure of the PSA.

About 27.4 % of histidine residues is located on the surface of the PSA molecule, while about 72.6 % of histidine residues is embedded in the interior region of the PSA molecule.