Trial to increase the sensitivity of brucella antigens treated with binary ethylene imine as inactivated agent

الملخص EN

The diagnosis of brucellosis is mainly based on the detection of anti-LPS antibodies.

High temperature kills BRUCELLA cells by causing lysis of the membrane, so the phenol-heat killed bracelet antigen may lake specificity as a result of destruction the majority of proteins in the cell wall.

Accordingly, attention was directed to produce antigen using binary ethylene imine as an in activator.

The produced antigen showed high specificity in detecting Brucella abort us and Brucella melitensis-infected animals, but sensitivity was not affected in comparison with the standard Rose Bengal antigen.

In Enzyme immunotransfer blot (EITB), phenol–heat killed bracelet cells showed only 3 bands (37.375, 23.47 and 7.83 kDa) that denote denaturation for at least 6 bands whereas binary inactivated bracelet cells showed similarity with non-treated ones.