Diagnosis of myopathies using histology, histochemistry, immunohistochemistry and electron microscopy : a single center experience
المؤلفون المشاركون
Owji, M.
Modarressi, F.
Alizadeh, A. R.
Heidari, T.
Gerami zadah, Bita
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 12، العدد 4 (31 يوليو/تموز 2010)، ص ص. 434-440، 7ص.
الناشر
تاريخ النشر
2010-07-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : skeletal muscle biopsy is important for the diagnosis of motor unit disorders, systemic diseases and metabolic disorders.
In some cases, routine histopathology methods are not conclusive and histochemistry, immunohistochemistry and even an electron microscopic study are required.
In this study, we describe our experience in the diagnosis of myopathies, considering all of the above-mentioned methods.
Methods : during a period of 18 months, 43 specimens of patients with the impression of myopathy were submitted to the Pathology Department and were evaluated with H & E and histochemical stainings (PAS, Oil red O, ATPase, NADH-TR, Gomorra Trachoma), immunohistochemistry (IHC) for dystrophic and electron microscopy.
Three specimens were excluded from the study because there were only adipose tissues and no adequate muscle was present for evaluation.
Results : Twenty three (57.5 %) males and 17 (42.5 %) females with a mean age of 34 years were evaluated.
The results were as follows : becker's muscular dystrophy (5 cases, 12.5 %), Duchene's muscular dystrophy (3 cases, 7.5 %), fascioscapulohumeral dystrophy (3 cases, 7.5 %), limb girdle dystrophy (2 cases, 5 %), polymyositis (6cases, 15 %), dermatomyositis (2 cases 5 %), McArdle's disease (1 case, 2.5 %), hypothyroidism myopathy (1 case, 2.5 %), type 2 atrophy secondary to drugs and systemic diseases (2 cases 12.5 %), congenital myopathy (2 cases 5 %), McArdle (1 case 2.5 %), unclassified myopathy (2 cases, 5 %), and normal muscle biopsy (8 cases, 20 %).
Although a genetic study was not available to confirm the diagnosis of cases such as fascioscapulohumeral myopathy, the diagnosis was made after putting all of the findings together including clinical presentation, family history, NCV, EMG, etc.
Conclusion : in the cases with no definite diagnosis by the histology, histochemistry and IHC, we should perform an EM study to find out the distinct ultra-structural changes which can be diagnostic for some muscle disorders.
EM study in conjunction with light microscopy of muscle biopsy could be very helpful in establishing the diagnosis of some types of myopathies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Owji, M.& Modarressi, F.& Gerami zadah, Bita& Alizadeh, A. R.& Heidari, T.. 2010. Diagnosis of myopathies using histology, histochemistry, immunohistochemistry and electron microscopy : a single center experience. Iranian Red Crescent Medical Journal،Vol. 12, no. 4, pp.434-440.
https://search.emarefa.net/detail/BIM-252565
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Owji, M.…[et al.]. Diagnosis of myopathies using histology, histochemistry, immunohistochemistry and electron microscopy : a single center experience. Iranian Red Crescent Medical Journal Vol. 12, no. 4 (Jul. 2010), pp.434-440.
https://search.emarefa.net/detail/BIM-252565
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Owji, M.& Modarressi, F.& Gerami zadah, Bita& Alizadeh, A. R.& Heidari, T.. Diagnosis of myopathies using histology, histochemistry, immunohistochemistry and electron microscopy : a single center experience. Iranian Red Crescent Medical Journal. 2010. Vol. 12, no. 4, pp.434-440.
https://search.emarefa.net/detail/BIM-252565
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 440
رقم السجل
BIM-252565
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر