Assessment of hypo-osmotic swelling test (HOST) of spermatozoa supplemented with pentoxifylline (PTX) after preparation by conventional layering technique in infertile patients undergoing intra-uterine insemination performance
العناوين الأخرى
تقييم فحص كفاءة الغشاء البلازمي للنطفة البشرية تحت الضغط التنافدي الواطئ بإضافة مادة البنتوكسفلين باستخدام النظرية الطباقية البسيطة و تقنية التلقيح الاصطناعي داخل الرحم لمرضى العقم
المؤلفون المشاركون
al-Rikabi, Basim Khamis Kuti
al-Zubaydi, Amir Hasan Abd al-Kazim
المصدر
العدد
المجلد 6، العدد 2 (31 مارس/آذار 2011)، ص ص. 38-45، 8ص.
الناشر
جامعة ذي قار قسم البحث و التطوير
تاريخ النشر
2011-03-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
The current study was designed to compare the percentage of human sperm hypo-osmotic swelling test (HOST) with and without pentoxifylline supplemented for semen samples prepared by conventional layering technique by using 1ml of the liquefied semen was layered beneath 1ml of IVF culture medium after intra-uterine insemination (IUI) technique.
Form each infertile male, Thirty normal semen samples were collected by masturbation after 3-51 days abstinence and allow liquefying at 37°C in 5% C02 for 30 minutes and evaluated before and after in vitro sperm activation.
The final samples were divided into two tubes, each one contain 0.5 ml liquefied semen.
Therefore, 0.5 ml pentoxifylline was added into one tube and another tube as a control, also hypo-osmotic swelling test (HOST) was evaluated for each | semen samples.
The hypo-osmotic swelling test (HOST) was performed before and after in I vitro sperm preparation by mixing 0.1 ml of liquefied semen with 1.0 ml of 150 mOsm/L NaCl as hypo-osmotic solution.
The sperm function tests (SFTs) including sperm concentration, sperm motility (%), progressive sperm motility (%), and normal sperm morphology (%) were evaluated according to World Health Organization (WHO) criteria.
The results of the present study indicate that the percentage of swollen spermatozoa in pentoxifylline supplemented group was significantly higher than the control group (70.80±2.32 vs.
58.75±1.58; P<0.001).
It was concluded that addition of pentoxifylline to the semen samples prepared by conventional layering technique can improve functional integrity of sperm plasma membrane.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Rikabi, Basim Khamis Kuti& al-Zubaydi, Amir Hasan Abd al-Kazim. 2011. Assessment of hypo-osmotic swelling test (HOST) of spermatozoa supplemented with pentoxifylline (PTX) after preparation by conventional layering technique in infertile patients undergoing intra-uterine insemination performance. University of Thi-Qar Journal،Vol. 6, no. 2, pp.38-45.
https://search.emarefa.net/detail/BIM-253329
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Rikabi, Basim Khamis Kuti& al-Zubaydi, Amir Hasan Abd al-Kazim. Assessment of hypo-osmotic swelling test (HOST) of spermatozoa supplemented with pentoxifylline (PTX) after preparation by conventional layering technique in infertile patients undergoing intra-uterine insemination performance. University of Thi-Qar Journal Vol. 6, no. 2 ( Mar. 2011), pp.38-45.
https://search.emarefa.net/detail/BIM-253329
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Rikabi, Basim Khamis Kuti& al-Zubaydi, Amir Hasan Abd al-Kazim. Assessment of hypo-osmotic swelling test (HOST) of spermatozoa supplemented with pentoxifylline (PTX) after preparation by conventional layering technique in infertile patients undergoing intra-uterine insemination performance. University of Thi-Qar Journal. 2011. Vol. 6, no. 2, pp.38-45.
https://search.emarefa.net/detail/BIM-253329
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 43-45
رقم السجل
BIM-253329
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر