Rapid decontamination of Gutta Percha cones using different chemical agents

الملخص EN

To compare the effectiveness of four different disinfectant solutions in rapid decontamination of gutta percha cones.

Materials and Methods : In this experimental study, 320 gutta percha cones were placed in bacterial suspensions of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Can-dida albicans for 30 minutes, and then immersed in disinfectant solutions (6% hydrogen peroxide, 2% chlorhexidine, 1% sodium hypochlorite, and 70% ethyl alcohol) for 1, 5, 10, and 15 minutes.

After that, the cones were aseptically transferred to the test tubes containing 4 ml brain heart infusion broth, and then cultured on selected agar media and number of colonies was estimated after 24 hours incubation at 37 oC.

Results: Hydrogen peroxide was effective in 1 minute, while chlorhexidine and sodium hypoch-lorite after 10 minutes, and ethyl alcohol in 5 minutes for decontamination of gutta percha cones.

Con-clusion : Hydrogen peroxide was the most effective agents in decontamination of gutta perch cones.