Extraction, purification and physicochemical properties of carcinoembryonic antigen (CEA)‎

الملخص EN

A procedure has been described for the purification of the CEA of the human mammary carcinoma.

Tumor tissue extraction in (0.6) M perchloric acid followed by Ion exchange and column chromatography on Sepharose CL–6B column resulted in highly purified CEA preparation as determined by both immunological and physicochemical criteria.

Such as, immunoelectrophoresis, stocks radius, isoelectric point, M.

wt., stability and carbohydrate content.

The collected data suggest that CEAs of mammary adenocarcinoma tissues with stocks radius (53 A°), pI (4) and the M.wt. have been found to be 175 KD. Finally purified CEA preparation gave rise to single band by disc SDS–polyacrylamide gel electrophoresis and a single preciptin arc against corresponding monoclonal anti–CEA on immunoelectrophoresis.

The purified CEA contained approximately 40 % carbohydrate through determination by phenol–sulfuric acid method.