A study of kinetic and thermodynamic on plasma ceruloplasmin in patients with bladder cancer

الملخص EN

The levels of plasma ceruloplasmin (CP) in patients with bladder cancer, bladder stones as pathological control and normal subjects were determined.

The study include the kinetic and thermodynamic properties.

The study include (35) patients suffer from bladder cancer, (25) patients suffer from bladder stones disease and (25) normal subjects.

The statistical analysis showed a significant increase (P < 0.05) in the activity of plasma CP in patients with bladder cancer as compared to pathological control and normal subjects, the optimum conditions of enzyme activity showed a 9.26 mmole / L concentration of pphenylene diamine (PPD) ( substrate) under incubation time of 15 min & 370C .

Estimation of some kinetic & thermodynamic parameters of plasma CP enzyme reaction, indicated that : the km values for the plasma CP were lower in bladder cancer patients than in normal subjects, while the Vmax values for the CP reaction found to be higher in bladder cancer patients than in normal, the forward reaction rate constant (k1) for CP decreased in bladder cancer patients.

The disease affected (increased) the half-life time (t1 / 2) of CP reactions, the Hill coefficient (n) values revealed that there was a significant difference in the case of bladder cancer patients as compared with those of the control groups, the activation energy Eact.

* for CP reaction decreased in bladder cancer patients compared with normal group subjects, the ΔH* values for CP enzyme in normal subjects and bladder cancer patients were positive that confirmed the reaction to be endothermic.

The ΔS* values for CP enzyme in normal subjects and bladder cancer patients were negative, whereas the ΔG* values for CP enzyme-substrate reactions in normal and bladder cancer patients groups were positive.