A comparative biochemical study of proteins profile in Iraqi children and adolescent with β–thalassemia

الملخص EN

The aim of the present research is to study different protein fractions in sera of children and adolescent with β-thalassemia major and minor and to compare the results with that of healthy control.

One hundred fifty children and adolescents were enrolled in this study, including 50 patients with β-thalassemia major, 50 patients with β-thalassemia minor as pathological control group and another apparently 50 healthy individuals as a control group.

The age of all studied groups ranged from (4-18) years.

Total protein, albumin and immunoglobulins were estimated in sera of all subjects.

A Significant decrease was found in the total protein and albumin levels in sera of both major and minor thalassemia patients compared to normal groups.

A Significant increase in immunoglobulin levels (IgG, IgM and IgA) was found in the sera of major and minor β-thalassemia patients compared to control.

Different protein parts in sera of all subjects were detected using cellulose acetate electrophoresis.

The results revealed significant reduction in β-globulin fractions in β-thalassemia major patients compared to the normal and pathological control groups.

Significant elevations in β-globulins fractions were observed in both major β-thalassemia and minor β-thalassemia as compared to normal control group.

As a Conclusion the alteration in some protein parts occurred which is more obvious in major thalassemia patients compared to the normal and pathological control groups.