Protective role of alcoholic extract of black currant (Vitis vinifera L.)‎ on renal function of adult male rats exposed to methionine overload and hydrogen peroxide

العناوين الأخرى

التأثير الوقائي للمستخلص الكحولي للزبيب الأسود (Vitis Vinifera L.)‎ على وظيفة الكلية في ذكور الجرذان البالغة المعرضة لفرط المثيونين و بيروكسيد الهيدروجين

المؤلفون المشاركون

al-Hashimi, Rusul Abd al-Hamid
Khudayr, Khalisah Kazim

المصدر

المجلة العراقية للعلوم البيطرية

الناشر

جامعة الموصل كلية الطب البيطري

تاريخ النشر

2009-12-31

دولة النشر

العراق

عدد الصفحات

9

التخصصات الرئيسية

الطب البيطري

الموضوعات

• الكيمياء
• الكلى
• النباتات الطبية
• الأمراض
• الكحول
• الجوانب الصحية

الملخص الإنجليزي

This study was designed to investigate the effect of methionine overload in male rats on induction of renal damage comparing to an oxidant (Hydrogen peroxide).It also aimed to study the protective role of alcoholic extract of black currant on some biological markers related with kidney function in methionine overload and 0.5% H2O2 treated male rats.

Fifty adult male rats were randomly divided into five equal groups (ten rats/ group) and were treated daily as follows for 42 days.

Rats in the first group (C) were received 0.5 ml of buffer (0.1M, PH 7) by oral intubations and served as control group.

Animals of the second group (T1) were received 0.5% H2O2 in drinking water, while rats of the third group (T2) were intubated orally 100 mg / kg B.W.

of D.L.

methionine diluting in buffer.

Animals of the fourth group (T3) were received 0.5% H2O2 in drinking water plus (60 mg / kg B.W) alcoholic extract of black currant, while animals of the fifth group (T4) were intubated with same previous concentration of methionine and alcoholic extract of black currant.

Fasting blood samples were collected from all experimental groups at 0, 21, and 42 days of experiment to study the following parameters: A- Serum glutathione (GSH) concentration (only at day 0 and 42).

B- Serum creatinine (SC) concentration.

C- Blood urea nitrogen (BUN) concentration.

D-Serum uric acid (SUA) concentration and E-BUN / SC ratio.

Sections of kidney were assessed for histopathological studies.

The result revealed that exposure of animals H2O2 in drinking water(T1) or methionine (T2) for six weeks caused significant decrease (P<0.05) in GSH concentration and significant increase (P<0.05) in SC, BUN, SUA concentrations and BUN /SC ratio as comparing to control (C).

The result also showed that animals treated with alcoholic extract of black currant plus either H2O2 (T3) or methionine (T4) showed significant decline in SC, BUN, SUA concentrations, and BUN/SC ratio with significant elevation of GSH concentration comparing to H2O2 (T1) and methionine (T2) treated groups.

Histological section of kidney exposed to 0.5 % H2O2 or methionine showed acute degenerative changes characterized by vacoulation of cytoplasm of epithelial cell lining tubule with infiltration of phagocytes and monocytes, while intubation of black currant in groups T3& T4 caused regression of renal damage induced by H2O2 or methionine.

It seems that 0.5% H2O2 was more effective than methionine in induction of oxidative stress and change in some biological markers related to kidney function.

Also it seems that alcoholic extract of black currant exert protective actions against the damaging effect of H2O2 and methionine.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-260683

• APA
• MLA
• AMA