Application of electerophoresis and immunoblot in diagnosis of pseudomonas aeruginosa isolated from respiratory system of camels

الملخص EN

This Study was carried out on 70 slaughtered camels.

210 samples (70 each of tracheal swabs, lungs and lymph nodes) for isolation of Pseudomonas aeruginosa.

Obtained results declared that Pseudomonas aeruginosa could be detected in 3 (4.28 %), 6 (4.28 %) and 3 (4.28 %) of the examined samples of tracheal swabs, lungs and lymph nodes respectively.

The in vitro antibiotic sensitivity tests for the isolated bacteria were discussed.

The results showed that Pseudomonas aeruginosa was sensitive to Gentamycin and Chloramphenicol, while this organism was resistant to Amikacin, Flucloxacillin, Penicillin, Neomycin and Ampicillin.

SDS- PAGE demonstrated that the protein fractions of Pseudomonas aeruginosa ranged between 62.123 KDa and 18.8 KDa.

Immunoblotting analysis of the OMPs of Pseudomonas aeruginosa demonstrated that the monoclonal antibody specific to Pseudomonas aeruginosa OMPs reacted with protein band at molecular weight of 28.017 KDa (more immunogenic band) while faint bands were revealed at 61.203 KDa, 46.148 KDa and 8.1954 KDa.