الإكثار الخضري الدقيق لنبات ألستروميرياrebecca صنف alstroemeria sp.

الملخص EN

In this study, a protocol for in vitro micropropagation of Alstroemeria c.v.

Rebecca was developed.

Different explants from shoots and rhizomes (after surface disinfection) were cultured on a solidified MS medium containing 30 g/l sucrose, 2 mg/l BAP and 0.2 mg/l NAA.

Results showed that the use of rhizome buds resulted in the highest growth percentage (100%) in the initial stage, followed by shoot tips (89.75 %) compared with the other explants.

The use of basal microcuttings obtained in the initial stage resulted in the highest multiplication rate (2.25).

In the multiplication stage, the cytokinin BAP produced higher multiplication rate and shoot length compared with kinetin.

The concentration 2 mg/l of BAP resulted in the highest multiplication rate (2.85) and shoot length (24.05 mm).

Treatment with 0.4 mg/l NAA resulted in the best rooting percentage (82.86 %) and the greatest root number (5.97).

The longest roots (49.36 mm) were obtained when IBA was used at a concentration of 0.1 mg/l.