G ATA-3 m RNA expressions in bronchial asthma effect of inhaled corticosteroid therapy

الملخص EN

GATA-3 is a T cdl-specific transcription factor and is expressed inTH2 but not TH1 cells.

It plays a critical role in TFE differentiation and allergic airway inflammation.

The aim of the work was to study non-invasive tool like induced sputum provide an accurate reflection of the inflammatory milieu in bronchial asthma as regards the expression of GATA-3 mRNA as well as the effect of indeed corticosteroid therapy on GATA-3 mRNA expression Methods : induced sputum samples were collected from46 subjects (10 healthy controls,18 roan topic and 18 atopic asthmatic patients).

Atop is based on positive family history and positive allergic skin test to group of common allergens.

For each sample, GATA-3 mRNA expression was erected by RT-PCR and the induced sputum eosinophilia percentage was determined.

Sputum unction was repeated for 10 Rona topic a 10 atopic patients after 6 weeks of indeed steroid therapy (Beclomethasone dipropiorate250 Ligtwicedaily).

Results : we demonstrated that 83 % of the studied untreated atopic asthmatic patients had increased expression of GATA-3 mRNA in their induced sputum samples In contrast, 61 % of tie studded untreated Ron atopic patients has decreased expression of GATA-3 mRNA in their induced sputum samples.

Fever, after long course of corticosteroid therapy both atopic and Ron atopic treated patients sow negative expression of GATA-3 mRNA Aitiorally, Terre were significant association between GATA-3 expression in both asthmatic groups before treatment and FEV1, blood eosinophilia count & nuked sputum eosinophilia percentage.

So, the surety of asthma could be Reflected from the mature of GATA-3 expression in the induced sputum Conclusion: Induced sputum provides sufficient sensitivity for examining transcription factors ; GATA-3 expressionism up regulated in atopic asthma.

Corticosteroids block the action of GATA-3 as a part of its effects.

Therefore, local delivery of antisense oligonucleotides blocking GATA-3 mRNA in atopic asthmatic patients will be at least as effective as the administration of corticosteroids.

In action, this will escape the numerous side effects of corticosteroids.