Methylene tetrahydrofolate reductase polymorphism as an atherosclerotic coronary risk factor among patients with coronary artery disease

الملخص EN

Background / Aim : recently several studies revealed discrepancies about the association of meth yenta trader of late reeducates (MTHFR) gene polymorphism and the development atherosclerotic coronary artery disease (ACAD) in different races and population.

The present study aimed to study MTHFR gene polymorphism among the controls and evaluate it as a risk factor for ACAD Egyptian patients.

Patients & Methods : polymerase chain reaction-restriction fragment length polymorphism analysis was used to detect the C677T variants of the MTHFR gene in 30 patients with ACAD versus 30 healthy controls.

Their serum homocysteine level was determined ELISA technique.

Fasting loud glucose, cretonne, cholesterol, high density lipoprotein cholesterol (HDL-C) and triglyceride were estimated y spectrophometric method, and low density lipoprotein cholesterol (LDL-C) was calculated.

Results : frequencies of MTHFR CC, CT and TT genotypes were 50 %, 30 % and 20 % in ACAD patient group versus 56.7 %, 26.6 % and 16.7 % in control.

Patients with ACAD showed significantly higher plasma homocyteine concentrations than control (12.03+3.4 Jamal /L VS 10.77 +1.9 Jamal / L, P < 0.05).

When patients and controls were classified according to their genotypes, no significant difference was found among the different genotypes in control group, as well as, between them and CC, CT type in patients group.

In contrast, patients with MTHFR TT genotype had significantly higher homocysteine concentration compared to the same genotype in control group (17.01 + 3.4 Jamal / L VS 11.1 + 0.89 M-mol / L).

Furthermore, they had higher serum cholesterol and lower HDL-C, which haveen correlated to their serum homocysteine concentrations.

Relative risk estimation revealed that patients with TT genotype were at greater risk than CT and CC genotypes.

Conclusion : it could be concluded that MTHFR TT genotype could be considered as a risk factor that could interact with other environmental factors and contribute to ACAD.