Comparative study of spectrophotometry and microbiological assay of azithromycin capsules

الملخص EN

The microcline azithromycin can be analyzed microbiologicaly and chemically using spectrophotometric assay in its pharmaceutical dosage forms.

Microbiological assay applying the agar diffusion method, for the determination of the antibiotic azithromycin was described.

Using a strain of Bacillus pumitus ATCC No.

8241 as the test organism, azithromycin at concentrations ranging from 1 to 4 µg / ml could be measured in capsules.

The method was linear (r2 > 0.99), precise, reproducible (coefficient of variation less than 10%) and accurate, the content percent of the tested drug was within the pharmacopoeial limit (95-105%).

The study developed a method for the assay of azithromycin in its pharmaceutical dosage forms using Lambda-2 UV / VIS spectro-photometric analysis at 225 nm band.

Azithromycin at concentrations ranging from 0.25 to 4 mg / ml and it is corresponding absorbance were used for calibration curve, and azithromycin samples at concentration of 0.5 mg / ml were measured in capsules.

All samples were found to be within the pharmacopoeial limit and the method was linear (r2 = 0.9998).

When the chemical method was compared with microbiological assay, statistically using the student t-test, it was found that there was no significant difference between the two methods in the determination of azithromycin in its pharmaceutical dosage forms (probability > 0.05).