Production of sheep anti triiodothyronine (t3)‎ antisera for devebpment of t3 ria kit

الملخص EN

This study was a trial to produce antisera against triiodothyronine (T3).

Small amounts of antibodies were detected due to a poor response of sheep to immunization.

Consequentely the amount of T3 tracer bound by the antibodies was found to be less than 10% with weak discrimination between zero and high standard doses in all bleeds which does not satisfy radioimmunoassay (RIA) technique requirements.

In this study, two local male Sudanese sheep (Ovis aries) were immunized with T3-immunogen intramuscularly and subcutaneously in different sites along their backs.

Sheep (I) was immunized with 25µg of antigen per Kg body weight in first and boosting injections, while the dose was only 10µg per Kg body weight for sheep (II).

T3-immunogen was emulsified in FCA for the first injection and in FIA for the boosting injections.

The sera obtained from both sheep after each injection were subjected to evaluation for the levels of circulating anti-T3 antibodies through both qualitative and quantitative tests.

The ests were performed for both purified and non-purified forms of antibodies with different separation techniques.

These methods include precipitation by second antibody assisted by polyethylene glycol (PEG), polystyrene beads, and liquid phase separation techniques.