Cd133 expression in colorectal adenocarcinoma an immunohistochemical and clinicopathological study

الملخص EN

Objective : Nowadays, the theory of cancer stem cells (CSC) driving tumor initiation is gaining wide support.

CSCs are increasingly realized to be necessary targets for effective cancer treatment.

The expression of CD133, a widely accepted CSC marker, has been studied in different malignancies.

Aim : We study the expression of CD133 in colorectal adenocarcinoma sections in relation to different clinic pathological parameters and compare two different immuno-histochemical scoring methods.

Materials and Methods : The study included 44 cases of colorectal adenocarcinoma.

Fourteen (31.8%) male and 30 (68.2%) female patients operated upon for colorectal carcinoma, with ages ranging between 26 and 80 years (mean 57.27 ± 14.08 years).

Immunohistochemical staining for CD133 was carried out on paraffin embedded tissue sections.

CD133 scoring was performed using both; the classic semi quantitative scoring and digital image analysis.

Results were statistically correlated with different clinic pathological parameters.

Results : 42 cases (95.5%) expressed CD133 with 50% recording high expression (in > 50% of tumor glands).

Semi quantitative CD133 scoring was significantly related to digital reaction density (p = 0.001) but not to area fraction (p = 0.07).

Semi quantitative CD133 scores were related to tumor grade (p = 0.03) and nodal invasion (p = 0.003) but not to depth of invasion (p = 0.19), gender (chi = 6.789, p = 0.34) or age (F = 2.043, P = 0.143).

Digital scores (area fraction and reaction density) were related to male gender (p = 0.004 and 0.02, respectively) and tumor grade (p = 0.008 and 0.005 respectively) but not to depth of invasion (p = 0.88 and 0.17 respectively) or nodal invasion (p= 0.72 and 0.54 respectively).

Morph metrically, nuclei of CD133 expressing tumor glands tended to be larger than negative glands (no statistical significance(.

Conclusions : CD133 is expressed in the vast majority of colorectal carcinoma cases.

Higher expression of CD133 correlates with tumor grade but not with depth of tumor invasion or length of progression free survival.

Morph metrically, nuclei of CD133 expressing tumor glands tend to be larger than those of negative glands.