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A study on in-vitro transdifferentiation of rat bone marrow stromal cells into neuroepithelial-like cells
المؤلفون المشاركون
Kaka, G. H. R.
Tiraihi, T.
Kheradmand, J. Arab
Delshad, A. R. Azizzadeh
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 11، العدد 2 (30 إبريل/نيسان 2009)، ص ص. 133-139، 7ص.
الناشر
تاريخ النشر
2009-04-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : bone marrow stem cells (BMSCs) are a rich source of stem cells and may represent a valid alternative to neural or embryonic stem cells by replacing the autologous damaged tissues in neurodegenerative diseases.
In this study, we attempted to devise a protocol for the induction of BMSCs into neuroepithelial-like cells (NELCs).
Methods : rat BMSCs were isolated from the long bones of adult Sprague–Dawley rats.
Their purity in the 4th passage was evaluated with fibronectin by immunocytochemistry, and the stemness marker Oct-4 was assessed by RT-PCR technique.
The cells were expanded and induced in the induction stage.
The BMSCs were incubated with either β-mercaptoethanol (βME) (1 mM), dimethyl sulfoxide (DMSO) (2 %) or biotylated hydroxyanisol or butylated hydroxyanisol (BHA) (200 μM) in α-MEM medium without fetal bovine serum (FBS).
They were washed with phosphate buffer saline (PBS) and proceeded to the 2nd phase of induction, where the induction medium was changed with α-MEM and 15% FBS containing all-trans retinoic acid (RA) (1 μM) (for 3 days).
Then, the expression of the markers was assessed with GFAP, nestin and neurofilament 68 antibodies, respectively and the expression of Oct-4 and NeuroD was evaluated by RT-PCR.
Results : the purity of the BMSCs at the 4th passage was more than 92%.
The mRNA of Oct-4 was expressed in these cells.
Induction of BMSCs by DMSO-RA could differentiate NELCs significantly more than βME-RA and BHA-RA.
The transdifferentiation of NELCs was evaluated by nestin antibody and NeuroD mRNA expression; later markers expressed very low detectable level in BMSCs.
But the differentiation of BMSCs into astrocytes was less in all of the experiment groups that is estimated GFAP antibody.
Conclusion : The application of DMSO-RA can transdifferentiate BMSCs into NELCs in-vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kaka, G. H. R.& Tiraihi, T.& Kheradmand, J. Arab& Delshad, A. R. Azizzadeh. 2009. A study on in-vitro transdifferentiation of rat bone marrow stromal cells into neuroepithelial-like cells. Iranian Red Crescent Medical Journal،Vol. 11, no. 2, pp.133-139.
https://search.emarefa.net/detail/BIM-27376
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kaka, G. H. R.…[et al.]. A study on in-vitro transdifferentiation of rat bone marrow stromal cells into neuroepithelial-like cells. Iranian Red Crescent Medical Journal Vol. 11, no. 2 (Apr. 2009), pp.133-139.
https://search.emarefa.net/detail/BIM-27376
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kaka, G. H. R.& Tiraihi, T.& Kheradmand, J. Arab& Delshad, A. R. Azizzadeh. A study on in-vitro transdifferentiation of rat bone marrow stromal cells into neuroepithelial-like cells. Iranian Red Crescent Medical Journal. 2009. Vol. 11, no. 2, pp.133-139.
https://search.emarefa.net/detail/BIM-27376
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 138-139
رقم السجل
BIM-27376
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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