A study of the possible protective role of onion and garlic volatile obls

الملخص EN

This study has put stress on the capability of onion and garlic oils as chemopreventive agents in relieving and detoxifying the aflatoxin Bl (AFBi) and carbon tetrachloride (CCU).

Both AFBi and CCI4 are known as carcinogenic agents.

The hepato-carcinogenicity effect was studied by evaluation of glutathione-S-hydrogenase and glutathione-S-transferase activity in liver tissues of rats treated with AFBi- CCI4.

This study was carried out on rats weighting 110-130g each.

The AFBi was used in a dose of 6 mg/kg b.

wt., CCI4 in a dose of 0.05 mg/kg b.wt.

and onion and garlic oils in a dose of600 mg/kg b.

wt.

Rats were treated for 16 weeks.

They were divided into 6 groups randomly as follows: ^ Group I: received saline and served as a control group.

Group II: rats were treated with AFBi for 4 weeks, then, CCI4 for 8 weeks, then, 4 weeks without any treatment.

Group III: rats were treated with the same regimen as in group II till the end of the 12 * week, then, they received onion oil for another 4 weeks.

Group IV: rats were treated with the same regimen as in group II till the end of the 12th week, then, they received garlic onion oil for another 4 weeks.

Group V: rats were treated with onion oil in the first 4 weeks and then treated with AFBi for 4 weeks followed by treatment with CCI4 for 8 weeks.

Group VI: rats were treated with garlic oil in the first 4 weeks and then treated with AFBi for 4 weeks followed by treatment with CCI4 for 8 weeks.

Treating rats with AFB1-CCI4 exerted gradual increase hi GSH level up to the twelfth week and then it tended to decrease by preventing further ASFB1-CCI4 application up to the sixteenth week.

The relative weights (% of body weight) of liver increased during the first 12 weeks in rats compared with the control group.

On the other hand, rats treated with onion and garlic oils before AFB1+CCI4 application depressed the increase of the relative weight of liver organ.

Data obtained clearly indicated that administration of both onion and garlic oils have relieved the liver damage induced by treating rats with AFB1+CCI4.

Oil samples diminished total bilirubin levels nearly to the normal level (control group), even when oil samples administrated before or after AFB1+CCI4 treatment, with the exception of onion oil samples fed after AFB1+CCI4 application, which exerted somewhat lower levels than control group.

A depressive effect of AFB1+CCI4 was noticed on all levels of total protein, albumin, globulin, and A/G ratio compared with the control group.

Either onion or garlic oils enhanced the accumulation of protein fraction, they effectively regulated A/G ratio after 16 weeks in rats treated with AFB1+CCI4.

In general, treating rats with either onion or garlic essential oils resulting in chemopreventive impacts, as decreased the elevation in both urea and creatinine concentrations throughout the experimental period (16 weeks).

Key words: Onion and garlic oils, AFBi, CC14, GSH, GST, Rat, liver, Kidney.