Effect of green tea on some biochemical parameters in rats treated with afb1-cc14 as cancer inducers

الملخص EN

In the present work 160 male albino rats were randomly divided into four groups (40 rats each): Group I (control group), Group II (AFB1-CCI4), Group HI (AFB1-CCI4, followed by green tea extract (GTE) as the sole source of drinking fluid and Group IV GTE followed by AFB1-CCI4.

The effect of GTE on the relative weight of liver and kidney, and some biochemical parameters were determined in rats treated with AFB1-CCI4.

Treating rats with AFB1+CCI4 (Group II) for 12 weeks increased the relative weight of liver together with kidney compared with thoseof untreated rats (Group I, control).

The enlargement of these organstended to decrease by the end of the experiment (16 weeks), but stillhigher than those of control.

The harmful effect of AFB1+CCI4 on therelative weight of the above mentioned organs, has been markedly remediated with applying GTE either after or before applying AFB1-CCI4.

Changes in ALT level in serum of rats of Group III was similar to those recorded in Group II during the first 12 week, but tend to decrease sharply to attend the levels recorded in serum of the animal of control group by the end of week 16.

Treating rats with GTE either after or before AFB1+CCI4 treatments depressed the level of ALT to values similar to those recorded in control animals (Group I).

The changes in AST in serum of rats of the four groups during the experimental period were similar to those demonstrated for ALT.

The serum total bilirubin in rats treated with AFBi followed by CCI4 Group II and III was significantly higher than those recorded for control animals especially during the first 12 weeks of the experiment.

Treatment with GTE before AFB1-CCI4 revealed total bilirubin levels very close to those observed for the control animals during the whole experemintal period.

Results indicated that both urea and creatinine (except for the first 2 weeks), increased steadily in rats treated with only AFB1+CCI4 (Group II and III) for 12 weeks, then their concentration tend to decrease during the last four weeks of the experiment.

Treating rats with GTE before AFB1+CCI4 treatment (Group IV) decreased the , elevation in both urea and creatinine concentration throughout the experimental period (16 weeks).

Pretreating with GTE seemed to be more effective as chemopreventive agents (Group IV) rather than post-treating (after AFBi+CCU) (Group III).

Data reveal that treating rats with AFB1+CCI4 (GroupII) resulted in gradual increases in GSH up to the twelfth week, and then it tended to decrease.

On the other hand, GST behaved in opposite trend shown in case of GSH.

Post-treating rats with GTE exerted such decreases in GSH values, but to less extent compared with GTE pretreated rats, which seemed to be more effective than post-treating GTE did.