In situ identification of sugar residues in monkeys salivary glands by lectin histochemsitry ii. parotid gland

الملخص EN

The parotid salivary gland (PSG) of the monkey was histochmically analyzed by lectin histochemistry aiming to investigate the features of the available sugar residues and their suggested biology.

These lectins were used as probe and the horseradish peroxidase (HRP) as visualant.

The intensity of lectin binding affinity of the secretory acini and the smaller excretory ducts (intercalated, striated and interlobular ducts) showed wide variations.

The reaction of the serous acini was strong with the Wheat gearm agglutinine (WGA), moderate with the Ulexus europius agglutinin (UEA-1), scanty with the Concanavalia ensiformes agglutinin (Con-A) and negative with Peanut agglutinin (PNA).

The lining epithelial cells of the intralobular ducts (intercalated and striated) showed intens reaction to WGA, Con-A and PNA while that of the interlobular duct was negative to all except the moderate reaction to PNA.

The goblet cells of the interlobular duct gave intense reaction with WGA only.

The present study suggested that, the nature and composition of stored glycoprotein’s in monkey PSG is heterogeneous.

The predominant terminal sugar residues is supposed to be α-D-GluNAc in secrertory acini and α-D-gluc and α-D-man in the ducts system.