IL-6 gene promoter polymorphism (G-174C)‎ and type2 diabetes in suez canal area

الملخص EN

Interleukin-6 (IL-6), a powerful inflammatory mediator, plays a pivotal role in the pathogenesis of insulin resistance and type 2 diabetes.

The IL-6 promoter polymorphism, at position -174 (G > C), has been associated to insulin sensitivity although contrasting data have been reported.

The aim of this study was to investigate the association of the IL-6 174 G > C promoter polymorphism with risk of T2DM in our populations.

PCR restriction fragments length polymorphism (PCR-RFLP) was used to determine the distribution of allele and genotype frequency of this variant.

We conducted a case–control study of 52 cases of type 2 diabetes (32 women and 20 men) and 32 control subjects (22 women and 10 men) from out patients diabetic clinic of Suez Canal hospital, age and gender-matched.

We evaluated the IL-6 -174 G > C genotype.

In control subjects, both C+ (CG + CC genotypes) and G- (GG genotype) carriers.

The genotypes of control subjects were 16 (50 %, G / G), 6 heterozygous (18.7 % G / C) and 10 homozygous (31.3 %, C / C).

In diabetic subjects, there were 26 subjects carriers of homozygous (GG, 50 %), 16 heterozygous (C / G, 30.8 %) and 10 homozygous (C / C, 19.2 %).There was significant difference in fasting blood glucose levels in control carriers of GG genotype compared to GG diabetic subjects' P < 0.05, and between control carriers of combining (GC+CC) genotype compared to (GC+CC) diabetic subjects' P < 0.05.

Also there were significant associations between IL6 polymorphisms and the risk of type 2 diabetes in women or men.

There were significant associations in lipids profile serum total cholesterol, s.

triglyceride, s.

low density lipoprotein and s.

high density lipoprotein between control GG genotype compared to GG diabetic subjects' P<0.05, also we found significant difference between control carriers of combining (GC+CC) genotype and (GC+CC) diabetic subjects P < 0.05.

Also we found higher non-significant biochemical parameters; triglyceride levels, total cholesterol and LDL cholesterol levels in control subjects carrying the G / G genotype comparing to subjects carrying the (GC+CC) genotype In addition, none of the IL6 polymorphisms was significantly associated with the age, sex and body mass index (BMI) in the control and diabetic subjects.

In summary, the data in this study support substantial associations between the common polymorphisms in IL6 gene and the risk of type 2 diabetes.