Detection of molecular markers of toxoplasmosis among Egyptian patients with miscarriage using avidity igg-elisa and western blotting

الملخص EN

A total of 54 miscarriage patients were divided into 3 groups.

GI: 10 toxoplasmosis patients with +ve IgM-ELISA ; GII : 24 toxoplasmosis patients with +ve IgG-ELISA, and G III: 20 non-toxoplasm-osis cross-matched females as a control.

All groups were subjected to IgG-avidity ELISA and IgG-avidity immunoblotting.

Avidity Indices (AI) by ELISA ranged from 22.6 % to 73.3 % in GI and from 9.6 %-75.6 % in GII.

AI were high (>40 %) in 3(30 %) patients in G I and in 8(33.3 %) patients in G II.

Sera of GI recognized the 20, 28,32,60,93 and 100Kda bands with 55 % reduction in the 38 and 60 Kda bands after treatment with 6 M urea solutions.

Sera of G II recognized the 20, 28, 32, 38, 45, 95-97and 106 Kda bands.

There was 12.5 %, 16.6 % and 16.7 % reduction in the 20, 32, and 106 Kda bands, respectively, after urea.

The 38 and 60 Kda bands were identified as good diagnostic mar-kers for the recent toxoplasmosis infection (GI).

The 20, 32 and 106 Kda bands were good markers of high avidity antibodies during the chronic toxoplasmosis (GII).