Oxidative criteria and somebone turnover markers in beta-thalassemic patients

الملخص EN

Bone disease in beta-thalassemia patients has multifactorial etiology ; increased iron stores and per-oxidative stress are involved factors.

This study aimed to investigate the relationship between some bone turnover markers and some oxidants, antioxidants parameters of betathalassemic patients.

The study included 50 patients were diagnosed as beta-thalassemia major aged between 3 and 40 years, 30 males and 20 females compared to 34 normal controls of same age and sex ratio.

The patients and the controls were divided into 2 groups : group A1 ≤ 18 years compared to their controls group B1 and group A2 > 18 years compared to their controls group B2.

All were subjected to full clinical examination and laboratory analysis of their blood and urine for bone turnover markers ; osteocalcin (OC), bone specific alkaline phosphatase (BAP) and urinary deoxypyridinoline (DPD).

Also parathormone (PTH), serum calcium (Ca) and phosphorus (Ph).

Oxidants and antioxidants determined were ; plasma malondialdehyde (MDA), nitric oxide (NO), blood reduced glutathione (GSH), erythrocyte Cu-Zn Superoxide dismutase (SOD).

Serum levels of copper (Cu), zinc (Zn), selenium (Se), vitamin E and total antioxidant capacity (TAOC) were also determined.

The results showed significant increase in urinary DPD and decrease in serum OC, BAP and PTH in thalassemia groups compared to controls.

Also, the results revealed significant elevation in plasma MDA, NO, SOD and serum Cu and TAOC especially in group A1.

While there was significant decrease in GSH, serum Zn, Se, and vitamin E.

Plasma OC was positively correlated with serum iron and NO and negatively correlated with plasma TAOC in thalassemia patients within age group 3 ≤ 18 years.

On other hand, plasma BAP level was positively correlated with the frequency of transfusion.

Moreover, a significant inverse correlation of plasma PTH with blood GSH and plasma Vit.E were observed in thalassemia patients within age group 18 ≤ 40.

Furthermore, a significant positive correlation of plasma OC with height, DPD with age and Ph.

were seen too in the same group.

Also, plasma DPD was negatively correlated with NO in thalassemia patients within age group 18 ≤ 40.

In Conclusion, there are disturbance in bone turnover markers in beta-thalassemia patients which favor increased bone resumption and decreased bone formation.

These markers have to some extent correlations with oxidant antioxidant parameters in those patients.