Association of HLA class ii alleles and CTLA-4 polymorphism with type 1 diabetes
المؤلفون المشاركون
Chmaisse, Hania Nakkash
al-Wafai, Rana J.
Makki, Rajaa F.
Fakhuri, Hana
المصدر
Saudi Journal of Kidney Diseases and Transplantation
العدد
المجلد 22، العدد 2 (30 إبريل/نيسان 2011)، ص ص. 273-281، 9ص.
الناشر
تاريخ النشر
2011-04-30
دولة النشر
السعودية
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
Type-1 diabetes mellitus (T1DM) is a progressive complex autoimmune disease in which combinations of environmental as well as genetic factors contribute to T-cell mediated destruction of insulin-secreting β-cells of the pancreas.
HLA class II alleles on chromosome 6p21 [insulin dependent diabetes mellitus 1 (IDDM1)], especially DR and DQ, show strong association with T1DM.
In addition, several studies have suggested that polymorphisms in the CTLA-4 gene (IDDM12) on chromosome 2q33 form part of the genetic susceptibility for type 1 diabetes.
The aim of this study was to analyze HLA alleles of the DQB1 and DRB1 genes using polymerase chain reaction using sequence specific primers (PCR-SSP) technique and to investigate the association of the A49G CTLA-4 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in Lebanese T1DM patients.
The study was conducted on 39 Lebanese T1DM patients.
Results of HLA typing showed an increased frequency of the HLA-DQB1*0201, HLA-DQB1*0302, HLA-DRB1*0301 and HLA-DRB1*0401 alleles, suggesting risk association and thus can be considered as susceptibility alleles.
On the other hand, strong protection against the disease was conferred by the HLA-DRB1*110101, HLA-DQB1*0301 and HLADQB1*0601 alleles.
RFLP analysis of the A49G polymorphism showed a significant increase in the G allele and GG genotype frequencies in patients, suggesting that CTLA-4 may be considered as a susceptibility gene for the development of T1DM in the Lebanese population.
Analysis of the two polymorphisms showed no detectable association between the two genes.
However, a significant negative association of the G allele with the DQB1*0201 allele was observed.
This might indicate that the two genetic risk factors, namely HLA and CTLA-4, act independently of each other with no additive effect.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Wafai, Rana J.& Chmaisse, Hania Nakkash& Makki, Rajaa F.& Fakhuri, Hana. 2011. Association of HLA class ii alleles and CTLA-4 polymorphism with type 1 diabetes. Saudi Journal of Kidney Diseases and Transplantation،Vol. 22, no. 2, pp.273-281.
https://search.emarefa.net/detail/BIM-291429
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Wafai, Rana J.…[et al.]. Association of HLA class ii alleles and CTLA-4 polymorphism with type 1 diabetes. Saudi Journal of Kidney Diseases and Transplantation Vol. 22, no. 2 (Apr. 2011), pp.273-281.
https://search.emarefa.net/detail/BIM-291429
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Wafai, Rana J.& Chmaisse, Hania Nakkash& Makki, Rajaa F.& Fakhuri, Hana. Association of HLA class ii alleles and CTLA-4 polymorphism with type 1 diabetes. Saudi Journal of Kidney Diseases and Transplantation. 2011. Vol. 22, no. 2, pp.273-281.
https://search.emarefa.net/detail/BIM-291429
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 280-281
رقم السجل
BIM-291429
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر