P15 (ink4b) and e-cadherin cpg island methylation is frequent in Egyptian acute myeloid leukemia
المؤلفون المشاركون
Muslim, Ghadah I.
al-Shakankiry, Nayara
المصدر
Journal of the Egyptian National Cancer Institute
العدد
المجلد 18، العدد 3 (30 سبتمبر/أيلول 2006)، ص ص. 227-232، 6ص.
الناشر
جامعة القاهرة المعهد القومي للأورام
تاريخ النشر
2006-09-30
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Hypermethylation within the promoters of selected genes is an epigenetic pathway that appears to be especially common in all types of human haematopoeitic neoplasms.
It is usually associated with inactivation of the involved genes, and can be reversed using demethylating agents.
The aim of this study is to evaluate the frequency of p15 and E-cadherin promoter methylation in Egyptian acute myeloid leukemia (AML) patients in an attempt to identify a subset of patients who might be candidates for demethylating agents as a form of targeted therapy either as a primary or as an adjunct to current standard induction and post-remission regimens.
Material and Methods : In the present work we have studied tumor-associated aberrant p15 and E-cadherin promotor methylation in 59 newly diagnosed acute myeloid leukemia (AML) patients using methylation specific PCR.
Results : Aberrant p15 promoter methylation was detected in 49 % (29 / 59) of the patients.
In 4 of these patients, no DNA could be amplified by the p15 unmethylated reaction showing a complete methylation of both alleles in the examined region.
In the remaining 25 cases both methylated and unmethylated DNA could be amplified.
Aberrant methylation of E-cadherin was detected in 63 % (37 / 59) of the cases.
In all of these cases both the methylated and the unmethylated alleles were amplified denoting partial methylation of the examined region.
Concomitant methylation of p15 and E-cadherin was detected in 40 % (23 / 59) of all the cases tested, while in 27 % (16 / 59) of the cases both genes were not methylated.
Conclusion : These results demonstrate that p15 and E-cadherin promoter methylation are frequent events in Egyptian AML and provide an impetus for larger studies to define the extent and pattern of methylation in the various subgroups of AML.
Methylation studies, therefore, represent a novel additional tool to define the subset of patients who might benefit from demethylating agents, thus providing the molecular basis for targeted therapeutic approaches and better designing of risk-adapted therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shakankiry, Nayara& Muslim, Ghadah I.. 2006. P15 (ink4b) and e-cadherin cpg island methylation is frequent in Egyptian acute myeloid leukemia. Journal of the Egyptian National Cancer Institute،Vol. 18, no. 3, pp.227-232.
https://search.emarefa.net/detail/BIM-29520
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shakankiry, Nayara& Muslim, Ghadah I.. P15 (ink4b) and e-cadherin cpg island methylation is frequent in Egyptian acute myeloid leukemia. Journal of the Egyptian National Cancer Institute Vol. 18, no. 3 (Sep. 2006), pp.227-232.
https://search.emarefa.net/detail/BIM-29520
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shakankiry, Nayara& Muslim, Ghadah I.. P15 (ink4b) and e-cadherin cpg island methylation is frequent in Egyptian acute myeloid leukemia. Journal of the Egyptian National Cancer Institute. 2006. Vol. 18, no. 3, pp.227-232.
https://search.emarefa.net/detail/BIM-29520
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : pp. 231-232
رقم السجل
BIM-29520
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر