Simultaneous determination of some pharmaceuticals in hospital effluents using HPLC with UV and fluorescence detectors

الملخص EN

The development and validation of an HPLC / UV / Fluorescence (FL) detection method is described, which enables the measurement of the most consumed pharmaceuticals (methotrexate, caffeine, diclofenac, glimepiride and ibuprofen) in Jordanian hospital effluents.

Separation was done on a RP-C8 column at a flow rate of 1 Ml / min using 1 : 1 H2O / acetonitrile with 0.1 % trifluoroacetic acid.

The samples (200 ml each) were extracted and cleaned-up on C18 cartridges.

Correlation coefficients of the pharmaceuticals calibration were higher than 0.997 using a UV detector and 0.996 using a fluorescence detector for methotrexate.

Recoveries were ranged from 87 % to 108.3 %.