Study of plasmid profile, susceptibility patterns of clinical staphylococcus aureus isolated from patients with otitis media in Basrah

الملخص EN

The present study was designed to investigate plasmid profile, the prevalence of multidrug resistant Staphylococcus aureus, which was done in Basrah, Iraq, during the period between September 2010 and January 2011.

A total of seventy six Ear swab were collected from seventy six patients (32 male and 44 female) with chronic and acute suppurative otitis media (CSOM, ASOM) with or without discharges.

Thirty five (46.02 %) isolates diagnosed as Staphylococcal spp and twenty four (31.6 %) as S.

aureus isolates from (10 male and 14 female).

S.

aureus isolates tested for antibiotic susceptibility, high antibiotic resistance to Ampicillin, Carbenicillin, Amoxicillin, Nalidixic acid, and Ceftriaxone , mid resistance to Streptomycin , Erythromycin and Oxacillin, low resistance to Neomycin, Tetracycline, Kanamycin,Tobramycin ,Gentamicin, Clinamycin, Cephalexin, and sensitive to Chloramphenicol, Vancomycin, Ciprofloxacin and Rifampicin .

S.aureus isolates showed multiple antibiotic resistances, such that, two isolates resisted four types of antibiotics.

Five isolates were resisted five types of antibiotics.

Three isolates resisted six types of antibiotics.

Five isolates resisted seven types of antibiotics.

Two isolates resisted eight types of antibiotics.

Two isolates resisted nine types of antibiotics.

Two isolates resisted ten types of antibiotics.

Two isolates resisted eleven types of antibiotics.

Only one isolate resisted fifteen types of antibiotics.

The MAR index of the isolates ranged between 0.2 and 0.75.

All isolates harbored Large molecular weight plasmids ranging from (21–22) Kbp.