Treatment of experimentally induced mastitis in nubian goats by staphylococcus aureus using methanolic extract of terminalia brownii leaves

الملخص EN

A series of experiments was designed to test the efficacy of the methanolic extracts of Terminalia brownii leaves in treating local infection in the udder of lactating Nubian goats.

Mastitis was induced experimentally in three groups of lactating goats (each group was of 5 goats) by the inoculation of pathogenic Staphylococcus aureus (Coagulase-positive strain) into each teat canal at the dose rate of 0.1ml of an over night broth culture, 1x10-3 containing 98-109 viable bacterial count per ml and 1x10-5 dilution of the same culture into the 3 groups of goats, respectively.

Methanolic extract of T.

brownii leaves at a concentration of 25 mg/ml, was infused at the dose rate of 1.5 ml daily into each teat canal of the infected udders for 12 days.

Leucocytes were counted every 3 days during the course of treatment and absence of the bacteria in the milk were used to assess the in vivo efficacy of the leaves extract in treating the induced S.

aureus mastitis in goats.

The methanolic extract of Terminalia brownii leaves treated successfully aureus induced mastitis in most goats of the three groups (40% and 60%).

That was apparent by the drop of leucocytes count in milk to the normal level and the absence of staph.

aureus colonies in cultures of milk specimens on blood agar.

KEY WORDS: Goat mastitis; Staphylococcus aureus; Terminalia brownii leaves extract; T.

brownii leaves extract in treatment of mastitis.