Expression of p16 protein and detection of Epstein Barr virus in malignant lymphoma

مقدم أطروحة جامعية

Dulaymi, Fatiha

مشرف أطروحة جامعية

Jaran, Adnan S.
Irshaid, Fawzi

أعضاء اللجنة

Khalaf, Maruf A.
al-Hadithi, Raji
al-Tarawinah, Khalid Ahmad

الجامعة

جامعة آل البيت

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

Malignant lymphoma is ranked 4th among all diagnosed cancer cases in Jordan for the year 2004.

Previous studies indicate that the pathogenesis of Hodgkin Lymphoma (HL) and several type of Non-Hodgkin Lymphoma (NHL) have been correlated with Epstein Barr Virus (EBV).

In addition, the p16 protein loss expression contributes to tumor progression in lymphoma.

Therefore our study aimed to detect the presence of EBV and to investigate the expression of p16 protein in HL and NHL.

A total of 55 patients' paraffin embedded tissues diagnosed with HL and NHL collected from the archives of the department of pathology at the King Hussein hospital, Amman, Jordan.

Samples were first grouped into HL and NHL.

30 patients accounting for 55 % presented with HL and 25 patients (45 %) presented with NHL.

HL were typed into 5 subtype according to World Health Organization (WHO) classification, there were 60 % (30 / 18) mixed cellularity, 24.33 % (7 / 30) nodular sclerosis, 6.66 % (2 / 30) lymphocyte depletion, 4.33 % (1 / 30) lymphocyte rich and 6.66 % (2 / 30) unclassified, the last three were grouped into one group called others with 5 cases.

According to International Working Formulation System, NHL is graded into high, intermediate and low grade with percentage of 48 % (12 / 25), 20 % (5 / 25) and 32 % (8 / 25) of the cases respectively.

The diagnosis was confirmed by staining the slides of these cases with Hematoxylin and eosin.

Immunohistochemistry staining (IHC) was also performed to study the expression of p16 protein and the presence of EBV in HL and NHL The expression of p16 protein and the detection of EBV in HL was observed in 35.33 % and 60 % of the cases respectively, a signification correlation was observed between the expression of p16 protein and HL (P < 0.05) and also there is a correlation between the presence of EBV and HL (P < 0.05).

Among NHL, the expression of p16 protein and the presence of EBV were detected in 84 % and 32 % of the cases respectively.

There is a significant correlation between the expression of p16 protein and NHL (P < 0.05), also between the presence of EBV and NHL (P < 0.05) but no significant correlation between the expression of p16 protein and the presence of EBV in both HL and NHL (p > 0.05).

In conclusion, no statistically significant correlation was observed between the expression p16 protein and the presence of EBV, but both p16 protein and EBV appear to play role in the pathogenesis of lymphoma.

التخصصات الرئيسية

الأحياء

عدد الصفحات

73

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-302878